2-FDCK HCL CAN BE FUN FOR ANYONE

The lab says fixation will lead to the dyes to leak with the cells. I feel the idea is always that fixation may possibly injury cell membranes. I did find a paper which describes fixation making use of methanol or acetic acid, right after staining which has a DCFDA by-product. DCFDA could be the reagent that stains ROS from the kit ab113851, and I